Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

41 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sequentially acting Sox transcription factors in neural lineage development [RNA-seq]

ABSTRACT: We report sequential binding but unique functions of different Sox transcription factors during distinct stages of neural differentiation Loss of function experiment for Sox2/Sox3 in neural progenitor cells and gene expression profile for Sox11 expressing (PSA NCAM sorted) early formed neurons.

ORGANISM(S): Mus musculus

SUBMITTER: Rickard Sandberg

PROVIDER: E-GEOD-33060 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Sequentially acting Sox transcription factors in neural lineage development.

Bergsland Maria M Ramsköld Daniel D Zaouter Cécile C Klum Susanne S Sandberg Rickard R Muhr Jonas J

Genes & development 20111115 23

Pluripotent embryonic stem (ES) cells can generate all cell types, but how cell lineages are initially specified and maintained during development remains largely unknown. Different classes of Sox transcription factors are expressed during neurogenesis and have been assigned important roles from early lineage specification to neuronal differentiation. Here we characterize the genome-wide binding for Sox2, Sox3, and Sox11, which have vital functions in ES cells, neural precursor cells (NPCs), and ...[more]

PMID: 22085726

Similar Datasets

Sequentially acting Sox transcription factors in neural lineage development

Project description:This SuperSeries is composed of the following subset Series: GSE33059: Sequentially acting Sox transcription factors in neural lineage development [ChIP-seq] GSE33060: Sequentially acting Sox transcription factors in neural lineage development [RNA-seq] GSE33061: Sequentially acting Sox transcription factors in neural lineage development [microarray] Refer to individual Series

2011-11-01 | E-GEOD-33024 | biostudies-arrayexpress

Sequentially acting Sox transcription factors in neural lineage development [ChIP-seq]

Project description:We report sequential binding but unique functions of different Sox transcription factors during distinct stages of neural differentiation Examination of genome-wide Sox2, Sox3 and Sox11 transcription factor binding during different stages of neurogenesis.

2011-11-01 | E-GEOD-33059 | biostudies-arrayexpress

Sequentially acting Sox transcription factors in neural lineage development [microarray]

Project description:We report sequential binding but unique functions of different Sox transcription factors during distinct stages of neural differentiation We used microarray to examine the molecular function of Sox3 in neural progenitor cells. Over-expression of Sox3 under Nestin-promoter in neural progenitor cells. Cells are transgenic for GFP in Sox1 locus and were FACS sorted to obtain pure populations.

2011-11-01 | E-GEOD-33061 | biostudies-arrayexpress

Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells

Project description:Improved Smart-Seq for sensitive full-length transcriptome profiling in single cells. Cells of four different origins were profiled using commercial SMARTer and compared to five variants of an improved protocol (Smart-Seq2).

2013-09-01 | E-GEOD-49321 | biostudies-arrayexpress

Evaluation of TRAP-sequencing technology with a versatile conditional mouse model

Project description:Evaluation of TRAP-sequencing by comparing transcriptomes and translatomes of different organs and distinct cell populations. E14.5 brain and kidney transcriptome and translatome profiling of organs or distinct cell populations from these organs was performed using the Illumina TruSeq RNA technology.

2013-10-28 | E-GEOD-51619 | biostudies-arrayexpress

Single cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs)

Project description:Single cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs) from three independent tonsil donors. Sequencing libraries were prepared from FACS sorted individual ILCs with the Smart-Seq2 protocol (Picelli et al. Nature Methods 2013)

2016-01-27 | E-GEOD-70580 | biostudies-arrayexpress

Sequentially acting Sox transcription factors in neural lineage development [microarray]

2011-11-01 | GSE33061 | GEO

Identification of Highly Conserved Putative Developmental Enhancers Bound by SOX3 in Neural Progenitors Using ChIP-Seq

Project description:SOX3 is highly expressed in neural progenitor cells (NPC) within the developing mouse cetral nervous system. A ChIP-Seq experiement was performed for SOX3 in NPCs derived from embryonic stem cells. Identification of SOX3 binidng sites from 3 independent ChIP-seq samples in NPCs

2014-08-31 | E-GEOD-57186 | biostudies-arrayexpress

Single-cell RNA-Seq reveals dynamic, random monoallelic gene expression in mammalian cells

Project description:In the diploid genome, genes come in two copies, which can have different DNA sequence and where one is maternal and one is paternal. In a particular cell, a gene could potentially be expressed from both copies (biallelic expression) or only one (monoallelic). We performed RNA-Sequencing on individual cells, from zygote to the cells of the late blastocyst, and also individual cells from the adult liver. Using first generation crosses between two distantly related mouse strains, CAST/Ei and C57BL/6, we determined the expression separately from the maternal and paternal alleles. We found that half of the genes were expressed by only one allele, randomly so that some cells would express the paternal allele, some the maternal and a few cell both alleles. We also observed the spread of the progressive inactivation of the paternal X chromosome. First generation mouse strain crosses were used to study monoallelic expression on the single cell level

2014-01-10 | E-GEOD-45719 | biostudies-arrayexpress

Sequentially acting Sox transcription factors in neural lineage development [RNA-seq]

Project description:We report sequential binding but unique functions of different Sox transcription factors during distinct stages of neural differentiation

2011-11-01 | GSE33060 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data