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Single cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs)

ABSTRACT: Single cell RNA-sequencing of human tonsil Innate lymphoid cells (ILCs) from three independent tonsil donors. Sequencing libraries were prepared from FACS sorted individual ILCs with the Smart-Seq2 protocol (Picelli et al. Nature Methods 2013)

ORGANISM(S): Homo sapiens

SUBMITTER: Asa Bjorklund

PROVIDER: E-GEOD-70580 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The heterogeneity of human CD127(+) innate lymphoid cells revealed by single-cell RNA sequencing.

Björklund Åsa K ÅK Forkel Marianne M Picelli Simone S Konya Viktoria V Theorell Jakob J Friberg Danielle D Sandberg Rickard R Mjösberg Jenny J

Nature immunology 20160215 4

Innate lymphoid cells (ILCs) are increasingly appreciated as important participants in homeostasis and inflammation. Substantial plasticity and heterogeneity among ILC populations have been reported. Here we have delineated the heterogeneity of human ILCs through single-cell RNA sequencing of several hundreds of individual tonsil CD127(+) ILCs and natural killer (NK) cells. Unbiased transcriptional clustering revealed four distinct populations, corresponding to ILC1 cells, ILC2 cells, ILC3 cells ...[more]

PMID: 26878113

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