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Genome-wide Analysis of Nascent Transcription in Saccharomyces cerevisiae

ABSTRACT: We combined the nuclear run-on (NRO) assay which labels and captures nascent transcripts with high throughput DNA sequencing to examine transcriptional activity in Saccharomyces cerevisiae. Examination of nascent transcripts and steady-state transcripts in exponentially growing and heat-shock treated yeast.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Carlos Araya

PROVIDER: E-GEOD-33136 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-Wide Analysis of Nascent Transcription in Saccharomyces cerevisiae.

McKinlay Anastasia A Araya Carlos L CL Fields Stanley S

G3 (Bethesda, Md.) 20111201 7

The assessment of transcriptional regulation requires a genome-wide survey of active RNA polymerases. Thus, we combined the nuclear run-on assay, which labels and captures nascent transcripts, with high-throughput DNA sequencing to examine transcriptional activity in exponentially growing Saccharomyces cerevisiae. Sequence read data from these nuclear run-on libraries revealed that transcriptional regulation in yeast occurs not only at the level of RNA polymerase recruitment to promoters but als ...[more]

PMID: 22384366

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