Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Soy LGI Protein NILs, seed fill

ABSTRACT: Evaluation of transcripts from soybean seed tissue during seed fill for a pair of near-isogenic lines contrasting in seed protein and oil and carrying an introgression at the linkage group I protein QTL region. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Yung-Tsi Bolon. The equivalent experiment is GM11 at PLEXdb.] genotype: LoProt - time: 25-50(3-replications); genotype: LoProt - time: >50-100(3-replications); genotype: LoProt - time: >100-200(3-replications); genotype: LoProt - time: >200-300(3-replications); genotype: HiProt - time: 25-50(3-replications); genotype: HiProt - time: >50-100(3-replications); genotype: HiProt - time: >100-200(3-replications); genotype: HiProt - time: >200-300(3-replications)

ORGANISM(S): Glycine max

SUBMITTER: Yung-Tsi Bolon

PROVIDER: E-GEOD-33408 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean.

Bolon Yung-Tsi YT Joseph Bindu B Cannon Steven B SB Graham Michelle A MA Diers Brian W BW Farmer Andrew D AD May Gregory D GD Muehlbauer Gary J GJ Specht James E JE Tu Zheng Jin ZJ Weeks Nathan N Xu Wayne W WW Shoemaker Randy C RC Vance Carroll P CP

BMC plant biology 20100303

<h4>Background</h4>The nutritional and economic value of many crops is effectively a function of seed protein and oil content. Insight into the genetic and molecular control mechanisms involved in the deposition of these constituents in the developing seed is needed to guide crop improvement. A quantitative trait locus (QTL) on Linkage Group I (LG I) of soybean (Glycine max (L.) Merrill) has a striking effect on seed protein content.<h4>Results</h4>A soybean near-isogenic line (NIL) pair contras ...[more]

PMID: 20199683

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Project description:TITLE: Transcriptional profiling of Rgene-mediated responses in rice PROJECT DESCRIPTION: The dominant gene Xa7 and the recessive gene xa5 of rice confer resistance to several races of the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). To reveal the modes of action and the defense responses these genes initiate, we decided to obtain the global transcriptional profiles of the rice cultivars IRBB7, IRBB5 (which harbor Xa7 and xa5, respectively) and IR24 undergoing early infection by the Xoo Race 2 strain PXO86. Both IRBB7 and IRBB5 are resistant to PXO86 (which carry the corresponding avirulence genes avrXa7 and avrxa5), whereas IR24 is susceptible. We inoculated by vacuum infiltration the three rice cultivars ten days after seed germination (or 2 weeks after sowing) and collected inoculated tissue at 5 different timepoints within the first day after inoculation. The transcriptional profiles obtained will provide valuable insight into the similarities and differences between incompatible interactions mediated by a dominant and a recessive Rgene, in comparison to a compatible interaction. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, David O Nino-Liu. The equivalent experiment is OS4 at PLEXdb.] genotype: IR24 - time: 0 hai(3-replications); genotype: IR24 - time: 3 hai(3-replications); genotype: IR24 - time: 6 hai(3-replications); genotype: IR24 - time: 12 hai(3-replications); genotype: IR24 - time: 24 hai(3-replications); genotype: IRBB5 - time: 0 hai(3-replications); genotype: IRBB5 - time: 3 hai(3-replications); genotype: IRBB5 - time: 6 hai(3-replications); genotype: IRBB5 - time: 12 hai(3-replications); genotype: IRBB5 - time: 24 hai(3-replications); genotype: IRBB7 - time: 0 hai(3-replications); genotype: IRBB7 - time: 3 hai(3-replications); genotype: IRBB7 - time: 6 hai(3-replications); genotype: IRBB7 - time: 12 hai(3-replications); genotype: IRBB7 - time: 24 hai(3-replications)

Dataset Information

Soy LGI Protein NILs, seed fill

Publications

Complementary genetic and genomic approaches help characterize the linkage group I seed protein QTL in soybean.

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