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Copy number variation profiling of gastric tissues: Cancer Samples vs. Matched Adjacent Noncancerous Samples

ABSTRACT: Copy number variation profiling of gastric tissues comparing gastric cancer tissues with matched adjacent noncancerous tissues. Goal was to determine the effects of chromosomal imbalances on gene expression and carcinogenesis or progression. 27 pairs of gastric tissues: gastric cancer tissues vs. matched adjacent noncancerous tissues.

ORGANISM(S): Homo sapiens

SUBMITTER: Cheng Lei

PROVIDER: E-GEOD-33428 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of genes with a correlation between copy number and expression in gastric cancer.

Cheng Lei L Cheng Lei L Wang Ping P Yang Sheng S Yang Yanqing Y Zhang Qing Q Zhang Wen W Xiao Huasheng H Gao Hengjun H Zhang Qinghua Q

BMC medical genomics 20120504

<h4>Background</h4>To elucidate gene expression associated with copy number changes, we performed a genome-wide copy number and expression microarray analysis of 25 pairs of gastric tissues.<h4>Methods</h4>We applied laser capture microdissection (LCM) to obtain samples for microarray experiments and profiled DNA copy number and gene expression using 244K CGH Microarray and Human Exon 1.0 ST Microarray.<h4>Results</h4>Obviously, gain at 8q was detected at the highest frequency (70%) and 20q at t ...[more]

PMID: 22559327

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