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Wide-scale analysis of alternative polyadenylation (APA) associated with proliferation and transformation using 3'-Seq

ABSTRACT: We mapped and quantified poly(A) sites in BJ and MCF10A cells under proliferative, arrested and transformed states Two human cell lines (BJ and MCF10A) examined under proliferative, arrested and transformed states

ORGANISM(S): Homo sapiens

SUBMITTER: Ran Elkon

PROVIDER: E-GEOD-33592 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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E2F mediates enhanced alternative polyadenylation in proliferation.

Elkon Ran R Drost Jarno J van Haaften Gijs G Jenal Mathias M Schrier Mariette M Oude Vrielink Joachim A F JA Agami Reuven R

Genome biology 20120702 7

<h4>Background</h4>The majority of mammalian genes contain multiple poly(A) sites in their 3' UTRs. Alternative cleavage and polyadenylation are emerging as an important layer of gene regulation as they generate transcript isoforms that differ in their 3' UTRs, thereby modulating genes' response to 3' UTR-mediated regulation. Enhanced cleavage at 3' UTR proximal poly(A) sites resulting in global 3' UTR shortening was recently linked to proliferation and cancer. However, mechanisms that regulate ...[more]

PMID: 22747694

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