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The Chimeric Oncoprotein TLS-CHOP Represses Expression of the Anticancer Cytokine MDA-7/IL-24 in Myxoid Liposarcoma Cells

ABSTRACT: The TLS-CHOP fusion protein is found in the majority of human myxoid liposarcomas (MLS), and is thought to have oncogenic functions. Until now, however, the molecular function of TLS-CHOP for oncogenesis is still elusive. In this report, we have revealed that knockdown of TLS-CHOP by specific siRNA in MLS-derived cell lines inhibits cell growth and leads to cell death. Thus, TLS-CHOP may be a promising therapeutic target for MLS treatment. Thus we explored the target genes of TLS-CHOP influence cell-proliferation or cell death with microarray. We compared the whole mRNA profiles of cells treated with TLS-CHOP siRNA or control oligo in two myxoid liposarcoma cell-line.

ORGANISM(S): Homo sapiens

SUBMITTER: Masami Tanaka

PROVIDER: E-GEOD-33616 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A novel oncogenic pathway by TLS-CHOP involving repression of MDA-7/IL-24 expression.

Oikawa K K Tanaka M M Itoh S S Takanashi M M Ozaki T T Muragaki Y Y Kuroda M M

British journal of cancer 20120515 12

<h4>Background</h4>Translocated in liposarcoma-CCAAT/enhancer binding protein homologous protein (TLS-CHOP) (also known as FUS-DDIT3) chimeric oncoprotein is found in the majority of human myxoid liposarcoma (MLS), but its molecular function remains unclear.<h4>Methods</h4>We knockdowned TLS-CHOP expression in MLS-derived cell lines by a specific small interfering RNA, and analysed the gene expression profiles with microarray.<h4>Results</h4>TLS-CHOP knockdown inhibited growth of MLS cells, and ...[more]

PMID: 22588557

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TLS-CHOP Fusion Oncoprotein Regulates MicroRNA-486 in Human Myxoid Liposarcoma

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