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The induction of integrin beta3 signaling in hematopoietic stem cells (HSCs)

ABSTRACT: To determine the molecules involved in beta3 integrin signaling in HSCs, we carried out DNA microarray analyses using HSCs stimulated with ligand-mimic antibody for intetegrin beta3 in the presence of stem cell factor(SCF) and/or thrombopoietin (TPO). All experiments were performed in duplicate or triplicate using sorted cells that were independently prepared in separate experiments. After CD34-KSL HSCs were cultured with 2C9.G2 or control IgG for 5 days in S-Clone SF-03 medium supplemented with 50 ng/ml SCF and/or 50 ng/ml TPO, gene expression profiles were examimed using 5,000 sorted CD48-KSL cells from each sample.

ORGANISM(S): Mus musculus

SUBMITTER: Terumasa Umemoto

PROVIDER: E-GEOD-33696 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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