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Role of de novo DNA methyltransferases in hematopoietic stem cells (RNA-seq)

ABSTRACT: To investigate the global transcriptome expression in Dnmt3ab dKO HSCs, we performed high-throughput sequencing of Poly A+ RNA (RNA-Seq) from purified HSCs (SP-KSL-CD150+). With biological duplicates, more than 200 million reads in total for each genotype of HSC were obtained. Mouse hematopoietic stem cell mRNA profiles of Dnmt3ab Dko HSCs, generated by deep sequencing, in duplicate, using Illumina Hiseq 2000

ORGANISM(S): Mus musculus

SUBMITTER: deqiang sun

PROVIDER: E-GEOD-50775 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dnmt3a and Dnmt3b have overlapping and distinct functions in hematopoietic stem cells.

Challen Grant A GA Sun Deqiang D Mayle Allison A Jeong Mira M Luo Min M Rodriguez Benjamin B Mallaney Cates C Celik Hamza H Yang Liubin L Xia Zheng Z Cullen Sean S Berg Jonathan J Zheng Yayun Y Darlington Gretchen J GJ Li Wei W Goodell Margaret A MA

Cell stem cell 20140814 3

Epigenetic regulation of hematopoietic stem cells (HSCs) ensures lifelong production of blood and bone marrow. Recently, we reported that loss of de novo DNA methyltransferase Dnmt3a results in HSC expansion and impaired differentiation. Here, we report conditional inactivation of Dnmt3b in HSCs either alone or combined with Dnmt3a deletion. Combined loss of Dnmt3a and Dnmt3b was synergistic, resulting in enhanced HSC self-renewal and a more severe block in differentiation than in Dnmt3a-null ce ...[more]

PMID: 25130491

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