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MeDIP-chip of zdp mutant

ABSTRACT: Using meDIP-chip to provide methylation profile in zdp mutant and identify hypermethylated regions comparing to wild type Arabidopsis plants. Examination of zdp mutant's methylome. 4 samples(1 mutant, 1 WT, each has two biological replicates)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jian-Kang Zhu

PROVIDER: E-GEOD-33719 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A DNA 3' phosphatase functions in active DNA demethylation in Arabidopsis.

Martínez-Macías María Isabel MI Qian Weiqiang W Miki Daisuke D Pontes Olga O Liu Yunhua Y Tang Kai K Liu Renyi R Morales-Ruiz Teresa T Ariza Rafael R RR Roldán-Arjona Teresa T Zhu Jian-Kang JK

Molecular cell 20120201 3

DNA methylation is an important epigenetic mark established by the combined actions of methylation and demethylation reactions. Plants use a base excision repair pathway for active DNA demethylation. After 5-methylcytosine removal, the Arabidopsis DNA glycosylase/lyase ROS1 incises the DNA backbone and part of the product has a single-nucleotide gap flanked by 3'- and 5'-phosphate termini. Here we show that the DNA phosphatase ZDP removes the blocking 3' phosphate, allowing subsequent DNA polyme ...[more]

PMID: 22325353

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