Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

61 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Deinococcus radiodurans R1: wildtype strains vs. DR0199-deletion strains

ABSTRACT: Transcriptional profiling of comparing wildtype strains with DR0199-deletion strains under normal growth conditions. One-condition experiment, wild type strains vs. DR0199-deletion strains. Three biological replicates were carried out.

ORGANISM(S): Deinococcus radiodurans

SUBMITTER: Yuejin Hua

PROVIDER: E-GEOD-33758 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Deinococcus radiodurans R1: rpob 9-bp-deletion vs. wild-type

Project description:Transcriptional profiling of comparing wildtype strains with rpob 9-bp-deletion strains under normal growth contidions. One-condition experiment, rpob 9-bp-deletion vs. wild-type. Two biological replicates were carried out.

2010-06-20 | E-GEOD-18661 | biostudies-arrayexpress

Deinococcus radiodurans R1: wildtype strains vs. DR1790-deletion strains

Project description:Transcriptional profiling of comparing wildtype strains with DR1790-deletion strains under normal growth contidions. Keywords: Genetic modification One-condition experiment, wild type strains vs. DR1790-deletion strains. Two biological replicates were carried out.

2010-09-10 | E-GEOD-22628 | biostudies-arrayexpress

Deinococcus radiodurans R1: wildtype strains vs. DR0199-deletion strains

Project description:Transcriptional profiling of comparing wildtype strains with DR0199-deletion strains under normal growth conditions.

2012-03-29 | GSE33758 | GEO

Deinococcus radiodurans R1: wildtype strains vs. DR1790-deletion strains

Project description:Transcriptional profiling of comparing wildtype strains with DR1790-deletion strains under normal growth contidions. Keywords: Genetic modification

2010-09-10 | GSE22628 | GEO

Deinococcus radiodurans R1: wildtype strains vs. oxyR disruption strains

Project description:In Deinococcus radiodurans, a previously unreported special characteristic of DrOxyR (DR0615) is found with only one conserved cysteine. dr0615 gene mutant is hypersensitive to H2O2, but a little to ionizing radiation. Site-directed mutagenesis and subsequent in vivo functional analyses revealed that the conserved cysteine (C210) is necessary for sensing H2O2, but its mutation did not alter the binding characteristics of OxyR on DNA. Under oxidant stress, DrOxyR is oxidized to sulfenic acid form, which can be reduced by reducing reagents. In addition, quantitative real-time PCR and global transcription profile results showed that OxyR is not only a transcriptional activator (e.g., katE, drb0125), but also a transcriptional repressor (e.g., dps, mntH). Transcriptional profiling of comparing wildtype strains with oxyR disruption strains under normal growth conditions. Keywords: Genetic modification

2008-02-01 | GSE9636 | GEO

Deinococcus radiodurans R1: rpob 9-bp-deletion vs. wild-type

Project description:Transcriptional profiling of comparing wildtype strains with rpob 9-bp-deletion strains under normal growth contidions.

2009-10-22 | GSE18661 | GEO

Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1.

Project description:The complete genome sequence of the radiation-resistant bacterium Deinococcus radiodurans R1 is composed of two chromosomes (2,648,638 and 412,348 base pairs), a megaplasmid (177,466 base pairs), and a small plasmid (45,704 base pairs), yielding a total genome of 3,284, 156 base pairs. Multiple components distributed on the chromosomes and megaplasmid that contribute to the ability of D. radiodurans to survive under conditions of starvation, oxidative stress, and high amounts of DNA damage were identified. Deinococcus radiodurans represents an organism in which all systems for DNA repair, DNA damage export, desiccation and starvation recovery, and genetic redundancy are present in one cell.

| S-EPMC4147723 | biostudies-literature

Preserving genome integrity: the DdrA protein of Deinococcus radiodurans R1.

Project description:The bacterium Deinococcus radiodurans can withstand extraordinary levels of ionizing radiation, reflecting an equally extraordinary capacity for DNA repair. The hypothetical gene product DR0423 has been implicated in the recovery of this organism from DNA damage, indicating that this protein is a novel component of the D. radiodurans DNA repair system. DR0423 is a homologue of the eukaryotic Rad52 protein. Following exposure to ionizing radiation, DR0423 expression is induced relative to an untreated control, and strains carrying a deletion of the DR0423 gene exhibit increased sensitivity to ionizing radiation. When recovering from ionizing-radiation-induced DNA damage in the absence of nutrients, wild-type D. radiodurans reassembles its genome while the mutant lacking DR0423 function does not. In vitro, the purified DR0423 protein binds to single-stranded DNA with an apparent affinity for 3' ends, and protects those ends from nuclease degradation. We propose that DR0423 is part of a DNA end-protection system that helps to preserve genome integrity following exposure to ionizing radiation. We designate the DR0423 protein as DNA damage response A protein.

| S-EPMC515370 | biostudies-literature

Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein.

Project description:Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing pathway, with splicing dependent on adjacent residues.

| S-EPMC151952 | biostudies-literature

Deinococcus radiodurans R1 Cells: Control vs. bphPR deletion mutant

Project description:Transcriptional profiling of Deinococcus radiodurans comparing control wild type cells with bphPR deletion mutant

2014-07-07 | GSE59135 | GEO