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Effect of Integrin-aBb2 on transcription in 2d or 3d.

ABSTRACT: M21 or M21L cells were grown either in 2d (on plastic) or in a 3d-collagen model. 2x2 design, in triplicate

ORGANISM(S): Homo sapiens

SUBMITTER: Martin Enge

PROVIDER: E-GEOD-34219 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Protein kinase Cα (PKCα) regulates p53 localization and melanoma cell survival downstream of integrin αv in three-dimensional collagen and in vivo.

Smith Stephen D SD Enge Martin M Bao Wenjie W Thullberg Minna M Costa Tânia D F TD Olofsson Helene H Gashi Behxhet B Selivanova Galina G Strömblad Staffan S

The Journal of biological chemistry 20120706 35

Protein kinase C α (PKCα) is overexpressed in numerous types of cancer. Importantly, PKCα has been linked to metastasis of malignant melanoma in patients. However, it has been unclear how PKCα may be regulated and how it exerts its role in melanoma. Here, we identified a role for PKCα in melanoma cell survival in a three-dimensional collagen model mimicking the in vivo pathophysiology of the dermis. A pathway was identified that involved integrin αv-mediated up-regulation of PKCα and PKCα-depend ...[more]

PMID: 22773839

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