Unknown,Transcriptomics,Genomics,Proteomics

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Profiling of transcripts in developing seeds of common bean (Phaseolus vulgaris) lines differing in major storage proteins


ABSTRACT: To understand the molecular basis for differences of common bean wild-type and mutant in sulphur amino acid content, transcripts were profiled at four developmental stages of seeds from wild-type SARC1 and major seed storage protein-deficient line SMARC1N-PN1 using a CustomArray 90K array. Microarray data confirmed that transcripts of storage and sulphur-rich proteins and sulphur-metabolism related genes were differentially expressed between the lines. The common bean (Phaseolus vulgaris) mutant line, SMARC1N-PN1 and its wild type, SARC1 used in the microarray experiment were grown in the field in London, ON, in 2009. Four developmental stages of seeds, based on fresh seed weight, were harvested. The stages of seeds used are Stage IV M-bM-^@M-^S cotyledon, 25 mg seed weight; Stage V M-bM-^@M-^S cotyledon, 50 mg seed weight; Stage VI M-bM-^@M-^S maturation, 150 mg seed weight, corresponding to the most active phase of reserve accumulation; and Stage VIII M-bM-^@M-^S maturation, 380 mg seed weight, corresponding to the onset of desiccation, were harvested for total RNA extraction. Four biological replicates for Stage IV and V and 3 biological replicates for Stage VI and VIII.

ORGANISM(S): Phaseolus vulgaris

SUBMITTER: Frederic Marsolais 

PROVIDER: E-GEOD-34233 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcripts of sulphur metabolic genes are co-ordinately regulated in developing seeds of common bean lacking phaseolin and major lectins.

Liao Dengqun D   Pajak Agnieszka A   Karcz Steven R SR   Chapman B Patrick BP   Sharpe Andrew G AG   Austin Ryan S RS   Datla Raju R   Dhaubhadel Sangeeta S   Marsolais Frédéric F  

Journal of experimental botany 20121012 17


The lack of phaseolin and phytohaemagglutinin in common bean (dry bean, Phaseolus vulgaris) is associated with an increase in total cysteine and methionine concentrations by 70% and 10%, respectively, mainly at the expense of an abundant non-protein amino acid, S-methyl-cysteine. Transcripts were profiled between two genetically related lines differing for this trait at four stages of seed development using a high density microarray designed for common bean. Transcripts of multiple sulphur-rich  ...[more]

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