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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Vicia narbonensis (legume) and Pisum sativum (pea) expressing a bacterial phosphoenol pyruvate carboxylase during seed maturation

ABSTRACT: In order to change assimilate partitioning during seed maturation a bacterial phosphoenolpyruvate carboxylase (PEPC) has been expressed in V. narbonensis under control of the LegB4 promoter. Transgenic bean seeds accumulate app. 20% more protein and have higher seed weight. Physiological and biochemical analyses indicate a shift of metabolic fluxes from sugars/starch to organic acids/free amino acids suggesting increased anaplerotic carbon flow. This indicates that the availability of carbon acceptors limits seed protein synthesis. Gene expression was analysed in growing embryos using macro-arrays containing 5,548 seed-specific pea genes. Radioactive labeled cDNA probes were prepared from RNA isolated from embryo of developing seeds of wild type and transgenic PEPC plants of V. narbonensis (15, 17, 19, 21, 25, and 30 DAP) and hybridized to cDNA macroarrays.

ORGANISM(S): Pisum sativum

SUBMITTER: Ruslana Radchuk

PROVIDER: E-IPKG-2 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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