Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from normal and MDS erythroids cell cutlures ex vivo


ABSTRACT: CD34 positive cells of bone marrow samples from normal and MDS samples were cultured ex vivo into erythroid conditions. We used microarrays to detail the gene expression programm of erythroid cells between normal and pathological (MDS) samples Mononuclear cells from bone marrow samples were selected on the expression of the CD34 membran marker. Then, they were cultured ex vivo during 14 days and total RNA samples were analyzed at day 7, 10 and/or 14 then compared between normal and MDS samples.

ORGANISM(S): Homo sapiens

SUBMITTER: olivier kosmider 

PROVIDER: E-GEOD-34268 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications


Normal human erythroid cell maturation requests the transcription factor GATA-1 and a transient activation of caspase-3, with GATA-1 being protected from caspase-3-mediated cleavage by interaction with the chaperone heat shock protein 70 (Hsp70) in the nucleus. Erythroid cell dysplasia observed in early myelodysplastic syndromes (MDS) involves impairment of differentiation and excess of apoptosis with a burst of caspase activation. Analysis of gene expression in MDS erythroblasts obtained by ex  ...[more]

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