Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of normal v early MDS v non-MDS anemia bone marrow CD34 cells to identify biomarkers of disease


ABSTRACT: Early, low risk IPSS (International Prognostic Scoring System) myelodysplasia (MDS) is a heterogeneous disorder where the molecular and cellular haematopoietic defects are poorly understood. To gain insight into this condition, we analyzed gene expression profiles of marrow CD34+ progenitor cells from normal karyotype, low blast count MDS patients, age-matched controls and patients with non-MDS anaemia. The aim of the study was to further understanding of the cellular defect in MDS and to identify biomarkers of disease Experiment Overall Design: Bone marrow (BM) CD34 cells were purified from patients with MDS, non-MDS anemia and from normal donors. Total RNA was extracted from Tri-reagent and quality verified on by capillary electrophoresis (Agilent). RNA was amplified by the Affymetrix small sample protocol. cRNA was hybridised to Affymetrix U133A chips under standard conditions. Initial data was analysed in MAS 5.0

ORGANISM(S): Homo sapiens

DISEASE(S): vitamin B12 deficiency

SUBMITTER: Alexander Sternberg 

PROVIDER: E-GEOD-2779 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Early, low-risk International Prognostic Scoring System (IPSS) myelodysplastic syndrome (MDS) is a heterogeneous disorder where the molecular and cellular hematopoietic defects are poorly understood. To gain insight into this condition, we analyzed gene expression profiles of marrow CD34+ progenitor cells from normal-karyotype, low-blast-count MDS patients, age-matched controls, and patients with non-MDS anemia. Given the heterogeneity of early MDS, a surprisingly consistent finding was decrease  ...[more]

Similar Datasets

2010-06-22 | E-GEOD-18911 | biostudies-arrayexpress
2012-02-14 | E-GEOD-34268 | biostudies-arrayexpress
2007-10-30 | E-GEOD-2666 | biostudies-arrayexpress
2010-03-24 | E-GEOD-19429 | biostudies-arrayexpress
2011-01-01 | E-MEXP-2878 | biostudies-arrayexpress
2007-08-30 | E-GEOD-2049 | biostudies-arrayexpress
2019-11-22 | PRD000749 | Pride
2013-11-05 | E-GEOD-46032 | biostudies-arrayexpress
2016-06-30 | E-GEOD-81372 | biostudies-arrayexpress
2016-06-30 | E-GEOD-81173 | biostudies-arrayexpress