Highly Parallel Genome-Wide Expression Analysis of Single Mammalian Cells (Optimization)
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Craig April
PROVIDER: E-GEOD-34364 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Highly parallel genome-wide expression analysis of single mammalian cells.
Fan Jian-Bing JB Chen Jing J April Craig S CS Fisher Jeffrey S JS Klotzle Brandy B Bibikova Marina M Kaper Fiona F Ronaghi Mostafa M Linnarsson Sten S Ota Takayo T Chien Jeremy J Laurent Louise C LC Loring Jeanne F JF Nisperos Sean V SV Chen Gina Y GY Zhong Jiang F JF
PloS one 20120208 2
<h4>Background</h4>We have developed a high-throughput amplification method for generating robust gene expression profiles using single cell or low RNA inputs.<h4>Methodology/principal findings</h4>The method uses tagged priming and template-switching, resulting in the incorporation of universal PCR priming sites at both ends of the synthesized cDNA for global PCR amplification. Coupled with a whole-genome gene expression microarray platform, we routinely obtain expression correlation values of ...[more]
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