Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome analysis of ROP10 GTPase-mediated abscisic acid (ABA) signaling in Arabidopsis


ABSTRACT: In this study, we have identified genes that are respectively activated and repressed by the low concentration of ABA and show that ROP10 gates a specific subset of genes that are responsive only to a low ABA concentration. Keywords: Whole seedlings of wild-type (Ws) and the mutant (rop10-1) treated with or without ABA 12 Arabidopsis ATH1 chips were purchased from Affymetrix. Total RNA was extracted and purified from 1µM ABA-treated or non-treated Ws and rop10-1. Each genotype/treatment has three biological replicates. Probe labeling, array hybridization and scanning were done by the University of California-Irvine DNA Microarray Facility.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Zhi-Liang Zheng 

PROVIDER: E-GEOD-3454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptome analysis reveals specific modulation of abscisic acid signaling by ROP10 small GTPase in Arabidopsis.

Xin Zeyu Z   Zhao Yihong Y   Zheng Zhi-Liang ZL  

Plant physiology 20051028 3


Abscisic acid (ABA) is a hormone that modulates a variety of agronomically important growth and developmental processes and various stresses responses, but its signal transduction pathways remain poorly understood. ROP10, a member of ROP small GTPases in Arabidopsis (Arabidopsis thaliana), is a plasma membrane-associated protein specifically involved in negative regulation of ABA responses. To dissect the ROP10-mediated ABA signaling, we carried out transcriptome analysis using the Arabidopsis f  ...[more]

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