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Transcriptome analysis of ROP10 GTPase-mediated abscisic acid (ABA) signaling in Arabidopsis

ABSTRACT: In this study, we have identified genes that are respectively activated and repressed by the low concentration of ABA and show that ROP10 gates a specific subset of genes that are responsive only to a low ABA concentration. Keywords: Whole seedlings of wild-type (Ws) and the mutant (rop10-1) treated with or without ABA 12 Arabidopsis ATH1 chips were purchased from Affymetrix. Total RNA was extracted and purified from 1µM ABA-treated or non-treated Ws and rop10-1. Each genotype/treatment has three biological replicates. Probe labeling, array hybridization and scanning were done by the University of California-Irvine DNA Microarray Facility.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Zhi-Liang Zheng

PROVIDER: E-GEOD-3454 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome analysis reveals specific modulation of abscisic acid signaling by ROP10 small GTPase in Arabidopsis.

Xin Zeyu Z Zhao Yihong Y Zheng Zhi-Liang ZL

Plant physiology 20051028 3

Abscisic acid (ABA) is a hormone that modulates a variety of agronomically important growth and developmental processes and various stresses responses, but its signal transduction pathways remain poorly understood. ROP10, a member of ROP small GTPases in Arabidopsis (Arabidopsis thaliana), is a plasma membrane-associated protein specifically involved in negative regulation of ABA responses. To dissect the ROP10-mediated ABA signaling, we carried out transcriptome analysis using the Arabidopsis f ...[more]

PMID: 16258012

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Transcriptome analysis of ROP10 GTPase-mediated abscisic acid (ABA) signaling in Arabidopsis

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2005-11-14 | GSE3454 | GEO

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2012-03-13 | E-GEOD-31593 | biostudies-arrayexpress

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2011-08-26 | E-GEOD-31664 | biostudies-arrayexpress

atmyc2_cop1_mutant_light_ABA_treatment

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2009-12-31 | E-GEOD-8955 | biostudies-arrayexpress

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2012-04-29 | E-GEOD-17002 | biostudies-arrayexpress

Transcript profiling of auxin/cytokinin crosstalk in the Arabidopsis primary root apex

Project description:AtIPT8/pga22 seedlings (gain-of-function mutant in Ws background; Sun et al. 2003, Plant Physiology 131, pp167-176) were grown on vertical plates for 7 days in LD. The seedlings were then incubated directly on the plate with medium containing 5 uM 17-beta-estradiol (for induction of the IPT8 gene) or 5 uM trans-zeatin for 12 and 24 h. 5 mm primary root tips were harvested from the seedlings and pooled for microarray analysis. Total RNA was isolated from the samples with the RNeasy Plant Mini Kit from Qiagen. 14 samples were used in this experiment.

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2006-09-01 | E-GEOD-5741 | biostudies-arrayexpress

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2010-06-16 | E-GEOD-16567 | biostudies-arrayexpress

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