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Elk1 Directs a Critical Component of Growth Signaling by the Androgen Receptor in Prostate Cancer

ABSTRACT: Elk1 directs selective gene induction that is a substantial and critical component of growth signaling by AR in PC cells. Gene expression in LNCaP cells was determined in androgen-depleted medium in response to R1881 in ELK1 knock down (via AR shRNA) or in cells treated with control shRNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Trumbly

PROVIDER: E-GEOD-34589 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The ETS domain transcription factor ELK1 directs a critical component of growth signaling by the androgen receptor in prostate cancer cells.

Patki Mugdha M Chari Venkatesh V Sivakumaran Suneethi S Gonit Mesfin M Trumbly Robert R Ratnam Manohar M

The Journal of biological chemistry 20130220 16

The androgen receptor (AR) is essential for diverse aspects of prostate development and function. Molecular mechanisms by which prostate cancer (PC) cells redirect AR signaling to genes that primarily support growth are unclear. A systematic search for critical AR-tethering proteins led to ELK1, an ETS transcription factor of the ternary complex factor subfamily. Although genetically redundant, ELK1 was obligatory for AR-dependent growth and clonogenic survival in both hormone-dependent PC and c ...[more]

PMID: 23426362

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Hormone-Independence of Prostate Cancer Cells is Supported by the Androgen Receptor without Binding to Classical Response Elements

Project description:Treatment of late passage (LP50) LNCaP cells with R1881 (androgen) and AR shRNA identified a gene program controlled by androgen receptor in the absence of androgen. Gene expression in late passage (LP50) LNCaP cells that had enhanced androgen-independent growth was determined in androgen-depleted medium in response to R1881 or AR knock down via AR shRNA

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siRNA ELK1 depletion and EGF stimulation

Project description:ELK1 is a well-known target of the ERK branch (EGF-responsive) of the MAP kinase pathway. This transcription profiling experiment studied the effects of ELK1 depletion by siRNA and subsequent EGF stimulation.

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Transcriptional profiling by array after knockdown of ERK2 and ELK1 in human embryonic stem cells

Project description:The ERK/MAPK signal transduction cascade is one of the key pathways regulating proliferation and differentiation in development and disease. In human embryonic stem cells (hESCs), ERK signaling is required for their self-renewing property. Here we studied the convergence of the ERK signaling cascade at the DNA by mapping genome-wide kinase-chromatin interactions for ERK2 in hESCs. We observe that ERK2 targets genes coding for small RNAs, histones, and genes involved in cellular metabolism and cell cycle. We find that the transcription factor ELK1 is essential in hESCs and that ERK2 co-occupies promoters bound by ELK1. Strikingly, promoters bound by ELK1 without ERK2 are occupied by Polycomb group proteins that repress genes involved in lineage commitment. In summary, we propose a model where extracellular signaling-stimulated proliferation and intrinsic repression of differentiation is integrated to maintain the identity of hESCs.

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Elk1 Directs a Critical Component of Growth Signaling by the Androgen Receptor in Prostate Cancer

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