Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Estrogen-responsive Estrogen Receptor alpha binding sites in MCF-7 by ChIP-seq


ABSTRACT: Identification of Estrogen Receptor alpha (ERa) binding sites by ChIP-seq in MCF-7 breast cancer cells following an estrogen treatment. This study describes molecular effects of estradiol treatment and subsequent regulation by ER for a single gene/locus. A public ER chipseq (available in SRA as ERR011973), in addition to our own data, guided us to regulatory regions were ER was binding that were then analyzed in detail using "manual" ChIP. MCF-7 cells were treated for 1 h either 10 nm estradiol (E2) or vehicle (ethanol) and subjected to ChIP using antibodies against ERa or IgG.

ORGANISM(S): Homo sapiens

SUBMITTER: Sami Heikkinen 

PROVIDER: E-GEOD-34759 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Proto-oncogene PIM-1 is a novel estrogen receptor target associating with high grade breast tumors.

Malinen Marjo M   Jääskeläinen Tiina T   Pelkonen Mikko M   Heikkinen Sami S   Väisänen Sami S   Kosma Veli-Matti VM   Nieminen Kaisa K   Mannermaa Arto A   Palvimo Jorma J JJ  

Molecular and cellular endocrinology 20121106 2


We searched ERα cistromes of MCF-7 breast cancer cells for previously unrecognized ERα targets and identified proto-oncogene PIM-1 as a novel potential target gene. We show that the expression of PIM-1 is induced in response to estradiol in MCF-7 cells and that the induction is mediated by ERα-regulated enhancers located distally upstream from the gene. In keeping with the growth-promoting role of the PIM-1, depletion of the PIM-1 attenuated the proliferation of the MCF-7 cells, which was parall  ...[more]

Similar Datasets

2012-12-17 | GSE34759 | GEO
2008-05-02 | GSE11317 | GEO
2008-06-11 | E-GEOD-11317 | biostudies-arrayexpress
2006-09-05 | GSE5405 | GEO
2021-10-13 | GSE166168 | GEO
2014-06-30 | E-GEOD-52351 | biostudies-arrayexpress
2014-06-30 | GSE57804 | GEO
2007-11-11 | E-GEOD-3529 | biostudies-arrayexpress
2014-06-30 | GSE52351 | GEO
2021-10-13 | GSE173976 | GEO