Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse tendon, cornea, and skin fibroblasts stimulated with mechanical stress to test the hypothesis that fibroblasts from different tissues are phenotypically distinct from one another.

ABSTRACT: In order to test the hypothesis that fibroblasts from different tissues are phenotypically distinct from one another, we have subjected tendon, skin and corneal fibroblasts of fetal mouse to mechanical stimulation by fluid flow and analyzed the transcriptional responses of the cells using Affymetrix MOE430 chip set containing two arrays MOE430A and MOE430B. Experiment Overall Design: The experiment is done using Affymetrix MOE430 chip set containing two arrays MOE430A - platform GPL339 and MOE430B - platform MOE340. Three biological replicates for tendon, skin and corneal fibroblasts subjected to mechanical stimulation as well as not stimulated (control) were analyzed yielding 18 samples per chip (36 in total).

ORGANISM(S): Mus musculus

SUBMITTER: Steve Winder 

PROVIDER: E-GEOD-3486 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Phenotypic responses to mechanical stress in fibroblasts from tendon, cornea and skin.

Mackley Jennifer R JR   Ando Joji J   Herzyk Pawel P   Winder Steven J SJ  

The Biochemical journal 20060601 2

Primary fibroblasts isolated from foetal mouse cornea, skin and tendon were subjected to linear shear stress and analysed for morphological parameters and by microarray, as compared with unstimulated controls. Approx. 350 genes were either up- or down-regulated by a significant amount, with 51 of these being common to all three cell types. Approx. 50% of altered genes in tendon and cornea fibroblasts were changed in common with one of the other cell types, with the remaining approx. 50% being sp  ...[more]

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