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Deep Sequence Analysis of non-small cell lung cancer: Integrated analysis of gene expression, alternative splicing, and single nucleotide variations in lung adenocarcinomas with and without oncogenic KRAS mutations

ABSTRACT: In this manuscript, we have used RNA-Sequencing experiment to obtain and integrate a variety of genomic features in order to identify signaling pathways that are associated to mutant KRAS lung tumors. 8 lung adenocarcinomas with mutant KRAS in lung tumors and 8 lung adenocarcarcinomas without KRAS mutation of which one sample is ran twice for QC purposes.

ORGANISM(S): Homo sapiens

SUBMITTER: Krishna Kalari

PROVIDER: E-GEOD-34914 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deep Sequence Analysis of Non-Small Cell Lung Cancer: Integrated Analysis of Gene Expression, Alternative Splicing, and Single Nucleotide Variations in Lung Adenocarcinomas with and without Oncogenic KRAS Mutations.

Kalari Krishna R KR Rossell David D Necela Brian M BM Asmann Yan W YW Nair Asha A Baheti Saurabh S Kachergus Jennifer M JM Younkin Curtis S CS Baker Tiffany T Carr Jennifer M JM Tang Xiaojia X Walsh Michael P MP Chai High-Seng HS Sun Zhifu Z Hart Steven N SN Leontovich Alexey A AA Hossain Asif A Kocher Jean-Pierre JP Perez Edith A EA Reisman David N DN Fields Alan P AP Thompson E Aubrey EA

Frontiers in oncology 20120210

KRAS mutations are highly prevalent in non-small cell lung cancer (NSCLC), and tumors harboring these mutations tend to be aggressive and resistant to chemotherapy. We used next-generation sequencing technology to identify pathways that are specifically altered in lung tumors harboring a KRAS mutation. Paired-end RNA-sequencing of 15 primary lung adenocarcinoma tumors (8 harboring mutant KRAS and 7 with wild-type KRAS) were performed. Sequences were mapped to the human genome, and genomic featur ...[more]

PMID: 22655260

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