Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Human colon cancer HT-29 and LS174T cells: stably transfected with shRNA-Ascl2/EGFP vs. stably transfected with shRNA-Control/EGFP


ABSTRACT: Achaete scute-like 2 (Ascl2), a basic helix-loop-helix (bHLH) transcription factor, controls the fate of intestinal stem cells. However, the role of Ascl2 in colon cancer progenitor cells remains unknown. The cell lines HT-29 (47.5-95% of CD133+ population) and LS174T (0.45% of CD133+ population) were chosen for functional evaluation of Ascl2 in colon cancer progenitor cells after gene knockdown by RNA interference. The microRNA (miRNA) microarrays identified 26 two-fold up-regulated miRNAs and 58 two-fold down-regulated miRNAs in shRNA-Ascl2/HT-29 cells and 178 two-fold up-regulated miRNAs and 172 two-fold down-regulated miRNAs in shRNA-Ascl2/LS174T cells. Two-condition experiment: shRNA-Ascl2/HT-29 cells vs. shRNA-Ctr/HT-29 cells, and shRNA-Ascl2/LS174T cells vs. shRNA-Ctr/LS174T cells. Biological replicates: 1 HT-29 cells stably transfected with shRNA-Ascl2/EGFP, 1 LS174T cells stably transfected with shRNA-Ascl2/EGFP, 1 HT-29 cells stably transfected with shRNA-Control/EGFP, and 1 LS174T cells stably transfected with shRNA-Control/EGFP, independently grown and harvested. One replicate per array.

ORGANISM(S): Homo sapiens

SUBMITTER: Rongquan Wang 

PROVIDER: E-GEOD-34926 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2012-01-07 | GSE34926 | GEO
| PRJNA150153 | ENA
2011-11-29 | E-GEOD-18560 | biostudies-arrayexpress
2014-10-22 | E-MTAB-1890 | biostudies-arrayexpress
2013-12-16 | E-GEOD-53338 | biostudies-arrayexpress
2012-09-13 | E-GEOD-40843 | biostudies-arrayexpress
2013-12-16 | E-GEOD-53337 | biostudies-arrayexpress
2010-07-07 | E-GEOD-20147 | biostudies-arrayexpress
2015-08-04 | E-GEOD-59369 | biostudies-arrayexpress
2015-08-05 | E-GEOD-49567 | biostudies-arrayexpress