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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profile of EHEC 86-24 fusK (HK) and fusR (RR) mutants

ABSTRACT: The goals of this experiment was to obtain a transcriptional profile of EHEC 86-24 upon deletion of fusK or fusR genes. The samples were grown in DMEM until late logaritmic phase then RNA was extracted using the TriZol method.

ORGANISM(S): Escherichia coli O157:H7

SUBMITTER: Alline Pacheco

PROVIDER: E-GEOD-34991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fucose sensing regulates bacterial intestinal colonization.

Pacheco Alline R AR Curtis Meredith M MM Ritchie Jennifer M JM Munera Diana D Waldor Matthew K MK Moreira Cristiano G CG Sperandio Vanessa V

Nature 20121118 7427

The mammalian gastrointestinal tract provides a complex and competitive environment for the microbiota. Successful colonization by pathogens requires scavenging nutrients, sensing chemical signals, competing with the resident bacteria and precisely regulating the expression of virulence genes. The gastrointestinal pathogen enterohaemorrhagic Escherichia coli (EHEC) relies on inter-kingdom chemical sensing systems to regulate virulence gene expression. Here we show that these systems control the ...[more]

PMID: 23160491

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Hfq virulence regulation in enterohemorhagic Escherichia coli O157:H7 strain 86-24

Project description:Escherichia coli O157:H7 is a food-borne pathogen that causes bloody diarrhea and hemolytic uremic syndrome. Hfq is an sRNA chaperone protein that is involved in post-transcriptional regulation of virulence genes in pathogenic bacteria. In EHEC strain EDL933, Hfq acts a negative regulator of the locus of enterocyte effacement (LEE) that encodes most of the proteins involved in type three secretion and attaching and effacing lesions. We deleted hfq in E. coli O157:H7 strain 86-24 and compared global transcription profiles of the hfq mutant to the wild type strain in exponential growth phase. Deletion of hfq affected transcription of genes common to nonpathogenic and pathogenic strains of E. coli as well as pathogen-specific genes. Downregulated genes in the hfq mutant included ler as well as genes encoded in LEE2-5 that encode for type three secretion and AE lesion formation. Decreased expression of the LEE genes in the hfq mutant occurred at mid-, late, and stationary growth phases in both LB and DMEM media as detected by qRT-PCR. We also confirmed decreased regulation of the LEE genes by examining secreted proteins and AE lesion formation by the hfq mutant and WT strains. Deletion of hfq also caused decreased expression of the two-component system qseBC involved in inter-kingdom signaling and virulence gene regulation in EHEC as well as an increase in stx2AB expression that encodes for the deadly Shiga toxin. Altogether, these data indicate that Hfq plays a different regulatory role in EHEC 86-24 from what has been reported for EHEC strain EDL933 and that the role of Hfq in EHEC virulence regulation extends beyond the LEE. Comparison of transcriptional regulation of the WT 86-24 isolate and the hfq mutant for the identification of regulated targets that were followed up by functional analysis.

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Project description:We aim to compare global transcriptomic analysis of wt and delta-nrdRmutant in Pseudomonas aeruginosa PAO1 during aerobic and anaerobic conditions.

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2013-01-08 | E-GEOD-43325 | biostudies-arrayexpress

Effects of sidA and AHL on EHEC virulence

Project description:A study on the effects of an sdiA mutant and the AHL molecule on the virulence of EHEC Comparison of wild type E. coli 8624 with and without AHL to and E. coli 8624 sdiA mutant with and without AHL to determine the effects of each factor on gene expression

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