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Identification of entertoxigenic Escherichia coli genes transcriptionally altered with the interaction of host cells

ABSTRACT: Genes identified in ETEC E24377A after interaction with host cells (Caco-2) after time points of 30 min, 60 min and 120 min. Both the adherent and non-adherent cells were profiled as well as E24377a grown only in tissue culture media. Each time and sample has at least duplicate samples

ORGANISM(S): Escherichia coli E24377A

SUBMITTER: David Rasko

PROVIDER: E-GEOD-40427 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional modulation of enterotoxigenic Escherichia coli virulence genes in response to epithelial cell interactions.

Kansal Rita R Rasko David A DA Sahl Jason W JW Munson George P GP Roy Koushik K Luo Qingwei Q Sheikh Alaullah A Kuhne Kurt J KJ Fleckenstein James M JM

Infection and immunity 20121031 1

Enterotoxigenic Escherichia coli (ETEC) strains are a leading cause of morbidity and mortality due to diarrheal illness in developing countries. There is currently no effective vaccine against these important pathogens. Because genes modulated by pathogen-host interactions potentially encode putative vaccine targets, we investigated changes in gene expression and surface morphology of ETEC upon interaction with intestinal epithelial cells in vitro. Pan-genome microarrays, quantitative reverse tr ...[more]

PMID: 23115039

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