Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of E.coli ydgG mutant vs. isogenic wild type in a mature biofilm


ABSTRACT: The E. coli Genechip antisense genome array was used to study the differential gene expression profile of E. coli K12 ydgG mutant compared to its isogenic wild type in a mature biofilm. Experiment Overall Design: Overnight cultures were diluted 100X into 1 L shake flasks containing 250 mL of LB + 0.2 % and 10 g of glass wool. The cells were shaken at 250 rpm and 37 C for 24 hours (final OD600=5 for the suspended cells), to form a biofilm on the glass wool, and RNA was isolated from the biofilm and Affymetrix protocol was used for preparing fragmented and labelled cDNA that was hybrizide to the chips.

ORGANISM(S): Escherichia coli

SUBMITTER: Thomas Wood 

PROVIDER: E-GEOD-3514 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

YdgG (TqsA) controls biofilm formation in Escherichia coli K-12 through autoinducer 2 transport.

Herzberg Moshe M   Kaye Ian K IK   Peti Wolfgang W   Wood Thomas K TK  

Journal of bacteriology 20060101 2


YdgG is an uncharacterized protein that is induced in Escherichia coli biofilms. Here it is shown that deletion of ydgG decreased extracellular and increased intracellular concentrations of autoinducer 2 (AI-2); hence, YdgG enhances transport of AI-2. Consistent with this hypothesis, deletion of ydgG resulted in a 7,000-fold increase in biofilm thickness and 574-fold increase in biomass in flow cells. Also consistent with the hypothesis, deletion of ydgG increased cell motility by increasing tra  ...[more]

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