Unknown,Transcriptomics,Genomics,Proteomics

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Development of miRNA expression signatures of MEFs deficient in a ER stress mediator ATF6a for tunicamycin treatment


ABSTRACT: To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator ATF6a were treated with tunicamycin for 12 or 24 hrs. miRNAs responsible for tunicamycin-treatment for 12hrs in ATF6a-dependent manner were extracted. Among them, expression of three miRNAs (miR-26a, miR-27b, miR-143) was quantified in the RNA samples from the same as the microarray by real-time PCR. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator ATF6a were treated with 2ug/mL tunicamycin for 12 or 24 hrs. Two independent experiments were performed at each time (untreated, 12 or 24 hrs). miRNAs responsible for tunicamycin-treatment for 12hrs in ATF6a-dependent manner were extracted.

ORGANISM(S): Mus musculus

SUBMITTER: Seiichi Oyadomari 

PROVIDER: E-GEOD-35176 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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