Genomics

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Development of miRNA expression signatures of MEFs deficient in a ER stress mediator ATF6b for tunicamycin treatment


ABSTRACT: To further development of our miRNA expression approach to ER stress, we have employed miRNA microarray expression profiling as a discovery platform to identify ER stress-responsible ones. Mouse embryonic fibroblasts (MEFs) deficient in a ER stress mediator ATF6b were treated with tunicamycin for 12 or 24 hrs. miRNAs responsible for tunicamycin-treatment for 12hrs in ATF6b-dependent manner were extracted. Among them, expression of three miRNAs (miR-15b, miR-20b, miR-92a) was quantified in the RNA samples from the same as the microarray by real-time PCR.

ORGANISM(S): Mus musculus

PROVIDER: GSE35177 | GEO | 2015/01/18

SECONDARY ACCESSION(S): PRJNA155989

REPOSITORIES: GEO

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