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Comparison of the effects of GMCSF, IL-4 and 15kDa Granulysin on elutriated monocytes: a 48 hour time point analysis

ABSTRACT: Gene expression profiling of elutriated monocytes cultured for 48 hours in RPMI-1640, 10% AB plamsa and one of the following: GMCSF (56IU/ml), IL-4 (2000 IU/ml), 15 kDa Granulysin (10 nM) Sources tested: monocytes form 3 donors tested at time 0, 4 hours, 12 hours, 24 hours, 48 hours 5 time points were compared: day 0 (cells before start the culture), 4, 12, 24, 48 hours

ORGANISM(S): Homo sapiens

SUBMITTER: Luciano Castiello

PROVIDER: E-GEOD-35304 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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15 kDa granulysin causes differentiation of monocytes to dendritic cells but lacks cytotoxic activity.

Clayberger Carol C Finn Michael W MW Wang Tianhong T Saini Reena R Saini Reena R Wilson Christine C Barr Valarie A VA Sabatino Marianna M Castiello Luciano L Stroncek David D Krensky Alan M AM

Journal of immunology (Baltimore, Md. : 1950) 20120514 12

Granulysin is expressed as two isoforms by human cytotoxic cells: a single mRNA gives rise to 15 kDa granulysin, a portion of which is cleaved to a 9 kDa protein. Studies with recombinant 9 kDa granulysin have demonstrated its cytolytic and proinflammatory properties, but much less is known about the biologic function of the 15 kDa isoform. In this study, we show that the subcellular localization and functions of 9 and 15 kDa granulysin are largely distinct. Nine kilodalton granulysin is confine ...[more]

PMID: 22586033

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Comparison of the effects of GMCSF, IL-4 and 15kDa Granulysin on elutriated monocytes: a 48 hour time point analysis

Project description:Gene expression profiling of elutriated monocytes cultured for 48 hours in RPMI-1640, 10% AB plamsa and one of the following: GMCSF (56IU/ml), IL-4 (2000 IU/ml), 15 kDa Granulysin (10 nM) Sources tested: monocytes form 3 donors tested at time 0, 4 hours, 12 hours, 24 hours, 48 hours

2012-05-19 | GSE35304 | GEO

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Homo sapiens

Project description:Comparison of the effects of GMCSF, IL-4 and 15kDa Granulysin on elutriated monocytes: a 48 hour time point analysis

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