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Transcription profiling of mouse Dnmt1 knockouts and TSA treated p53-/- and p53-/-Dnmt1-/- cells to investigate the role of DNA methylation in stable gene repression

ABSTRACT: The experiment was designed to study the effects of the Dnmt1 gene knockout on gene expression, as well as to analyze the effects of TSA treatment on both WT (p53-/-) and knockout (p53-/-Dnmt1-/-) cells ; The effect of TSA treatment on knockout and WT cells was tested at two different time points (24 and 48 hs), as well as two weeks after removal of TSA Experiment Overall Design: Seven hybridizations were performed: 1. p53-/- untreated (control), 2. p53-/- +TSA harvested after 24hs of exposure to TSA, 3. p53-/- +TSA 48hs, 4. p53-/-Dnmt1-/- untreated, 5.p53-/-Dnmt1-/- +TSA 24hs 6.p53-/-Dnmt1-/- +TSA 48hs 7.p53-/-Dnmt1-/- two weeks after removal of TSA

ORGANISM(S): Mus musculus

SUBMITTER: Laura Lande-Diner 

PROVIDER: E-GEOD-3534 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A large fraction of the animal genome is maintained in a transcriptionally repressed state throughout development. By generating viable Dnmt1(-)(/)(-) mouse cells we have been able to study the effect of DNA methylation on both gene expression and chromatin structure. Our results confirm that the underlying methylation pattern has a profound effect on histone acetylation and is the major effector of me-H3(K4) in the animal genome. We demonstrate that many methylated genes are subject to addition  ...[more]

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