Unknown,Transcriptomics,Genomics,Proteomics

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Microbiota-dependent gene expression in small intestinal epithelial cells (IECs)


ABSTRACT: Intestinal epithelial cells were isolated from total small intestine of each four 28-day old conventionally raised (conv) and germ-free (GF) bred C57BL/6 mice (protocol according to: Lotz et al., J. Exp Med. 2006). Total RNA was isolated by TriZol and ist purity was examined using a Bioanalyszer. We used microarrays to comparatively detail the global gene expression in primary total isolated intestinal epithelial cells. Four biological replicates from isolated intestinal epithelial cells obtained from each 4 germ-free bred and conventionally raised mice. Colour change.

ORGANISM(S): Mus musculus

SUBMITTER: Mathias Hornef 

PROVIDER: E-GEOD-35597 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Age-dependent TLR3 expression of the intestinal epithelium contributes to rotavirus susceptibility.

Pott Johanna J   Stockinger Silvia S   Torow Natalia N   Smoczek Anna A   Lindner Cornelia C   McInerney Gerald G   Bäckhed Fredrik F   Baumann Ulrich U   Pabst Oliver O   Bleich André A   Hornef Mathias W MW  

PLoS pathogens 20120503 5


Rotavirus is a major cause of diarrhea worldwide and exhibits a pronounced small intestinal epithelial cell (IEC) tropism. Both human infants and neonatal mice are highly susceptible, whereas adult individuals remain asymptomatic and shed only low numbers of viral particles. Here we investigated age-dependent mechanisms of the intestinal epithelial innate immune response to rotavirus infection in an oral mouse infection model. Expression of the innate immune receptor for viral dsRNA, Toll-like r  ...[more]

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