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Effect of ligase on T7 based RNA linear amplification


ABSTRACT: Zhao et al. Amplification Table 3 This experiment was designed to evaluate the effect of ligase used in the second strand cDNA synthesis on the fidelity of T7 based RNA linear amplification. BC2 total RNA was amplified with or without ligase using Affymetrix protocol. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Computed

ORGANISM(S): Homo sapiens

SUBMITTER: Hongjuan Zhao 

PROVIDER: E-GEOD-3561 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis.

Zhao Hongjuan H   Hastie Trevor T   Whitfield Michael L ML   Børresen-Dale Anne-Lise AL   Jeffrey Stefanie S SS  

BMC genomics 20021030 1


<h4>Background</h4>T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinomas and high-density cDNA microarrays.<h4>Results</h4>Using an optimized protocol, the average correlation coefficient of gene expression of 11,123 cDNA clones between amplified and unamplified  ...[more]

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