Unknown,Transcriptomics,Genomics,Proteomics

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High-throughput sequencing of endogenous small RNAs from rat vascular smooth muscle cells with and without Angiotensin II stimulation


ABSTRACT: Angiotensin II (Ang II)-mediated vascular smooth muscle cells (VSMC) dysfunction plays a critical role in cardiovascular diseases. However, the role of microRNAs (miRNAs) in this process is unclear. We used small RNA deep sequencing to profile Ang II-regulated miRNAs in rat VSMC and evaluated their role in VSMC dysfunction. Sequencing results revealed several Ang II-responsive miRNAs and bioinformatics analysis showed that their predicted targets can modulate biological processes relevant to cardiovascular diseases. Examined 4 samples of Rat VSMC. Control (without Ang II treatment) and 3 samples treated with Ang II for 1h, 3h, and 24h. Compared the changes in gene expression in Ang II treated samples relative to control samples.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Nancy Chen 

PROVIDER: E-GEOD-35664 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Small RNA sequencing reveals microRNAs that modulate angiotensin II effects in vascular smooth muscle cells.

Jin Wen W   Reddy Marpadga A MA   Chen Zhuo Z   Putta Sumanth S   Lanting Linda L   Kato Mitsuo M   Park Jung Tak JT   Chandra Manasa M   Wang Charles C   Tangirala Rajendra K RK   Natarajan Rama R  

The Journal of biological chemistry 20120319 19


Angiotensin II (Ang II)-mediated vascular smooth muscle cell dysfunction plays a critical role in cardiovascular diseases. However, the role of microRNAs (miRNAs) in this process is unclear. We used small RNA deep sequencing to profile Ang II-regulated miRNAs in rat vascular smooth muscle cells (VSMC) and evaluated their role in VSMC dysfunction. Sequencing results revealed several Ang II-responsive miRNAs, and bioinformatics analysis showed that their predicted targets can modulate biological p  ...[more]

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