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Global comparative transcriptome analysis of cartilage formation in vivo

ABSTRACT: We set out to characterize the gene expression changes which take place during chondrogenesis in the developing mouse limb. RNA derived from pre-condensed mesenchyme, mesenchymal condensations, and cartilage anlagen representing the earliest stages of tibial and fibular development was analysed by whole genome microarray analysis, and revealed 931 genes differentially expressed in these tissues. Among them were 892 genes not previously identified during the initation of chondrogenesis, including members of the Bmp, Wnt, Gdf, Sox, and Fox gene families. These microarray data were validated by qPCR, in situ hybridisation, and analysis of numerous genes already implicated in chondrogenesis in the scientific literature. 231 sections from a total of four 11.5dpc mouse hind limbs, three 12.5dpc mouse hind limbs, and four 13.5dpc mouse hind limbs (all from separate mice) were microdissected, and tissues from each time point were pooled. Thus, this experiment consisted of one replicate only.

ORGANISM(S): Mus musculus

SUBMITTER: Trevor Cameron 

PROVIDER: E-GEOD-35669 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global comparative transcriptome analysis of cartilage formation in vivo.

Cameron Trevor L TL   Belluoccio Daniele D   Farlie Peter G PG   Brachvogel Bent B   Bateman John F JF  

BMC developmental biology 20090310

<h4>Background</h4>During vertebrate embryogenesis the initial stages of bone formation by endochondral ossification involve the aggregation and proliferation of mesenchymal cells into condensations. Continued growth of the condensations and differentiation of the mesenchymal cells into chondrocytes results in the formation of cartilage templates, or anlagen, which prefigure the shape of the future bones. The chondrocytes in the anlagen further differentiate by undergoing a complex sequence of m  ...[more]