Unknown,Transcriptomics,Genomics,Proteomics

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Tiled Array Experiment of Murine Gammaherpesvirus 68 Transcripts In Newly Infected Fibroblasts


ABSTRACT: Murine NIH3T3 fibroblasts were infected with MHV68 and RNA expression was examined over an 18 hour timecourse experiment. Study includes 8 samples isolated at 0, 2, 4, 6, 8, 10, 12, and 18 hours after infection of NIH3T3 fibroblasts with murine gammaherpesvirus 68 at a multiplicity of infection of 10.0.

ORGANISM(S): Mus musculus

SUBMITTER: Jizu Zhi 

PROVIDER: E-GEOD-35863 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Tiled microarray identification of novel viral transcript structures and distinct transcriptional profiles during two modes of productive murine gammaherpesvirus 68 infection.

Cheng Benson Yee Hin BY   Zhi Jizu J   Santana Alexis A   Khan Sohail S   Salinas Eduardo E   Forrest J Craig JC   Zheng Yueting Y   Jaggi Shirin S   Leatherwood Janet J   Krug Laurie T LT  

Journal of virology 20120208 8


We applied a custom tiled microarray to examine murine gammaherpesvirus 68 (MHV68) polyadenylated transcript expression in a time course of de novo infection of fibroblast cells and following phorbol ester-mediated reactivation from a latently infected B cell line. During de novo infection, all open reading frames (ORFs) were transcribed and clustered into four major temporal groups that were overlapping yet distinct from clusters based on the phorbol ester-stimulated B cell reactivation time co  ...[more]

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