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Transcript profiling of Arabidopsis msh1 mutants

ABSTRACT: The Arabidopsis msh1 mutant show various growth phenotype. We use microarray analysis to characterize gene expression pattern for two of the phenotypes - variegation and stunted growth. Wild type and msh1 RNAi variegated and stunted tissues were collected for RNA extration and hybridization on Affymetrix.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ying-Zhi Xu

PROVIDER: E-GEOD-35893 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The chloroplast triggers developmental reprogramming when mutS HOMOLOG1 is suppressed in plants.

Xu Ying-Zhi YZ Santamaria Roberto de la Rosa Rde L Virdi Kamaldeep S KS Arrieta-Montiel Maria P MP Razvi Fareha F Li Shaoqing S Ren Guodong G Yu Bin B Alexander Danny D Guo Lining L Feng Xuehui X Dweikat Ismail M IM Clemente Tom E TE Mackenzie Sally A SA

Plant physiology 20120409 2

Multicellular eukaryotes demonstrate nongenetic, heritable phenotypic versatility in their adaptation to environmental changes. This inclusive inheritance is composed of interacting epigenetic, maternal, and environmental factors. Yet-unidentified maternal effects can have a pronounced influence on plant phenotypic adaptation to changing environmental conditions. To explore the control of phenotypy in higher plants, we examined the effect of a single plant nuclear gene on the expression and tran ...[more]

PMID: 22496509

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High-throughput Illumina sequencing of bisulfite transformed genomic DNA from Arabidopsis thaliana wild-type, msh1 mutant and MSH1-epiF3 lines

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High-throughput Illumina sequencing of bisulfite transformed genomic DNA from Arabidopsis thaliana wild-type, msh1 mutant and MSH1-epiF3 lines

Project description:Arabidopsis msh1 mutants display developmental reprogramming (dr) phenotypes, include reduction in growth, enhanced branching, and delayed maturation and flowering time. MSH1-epi lines were derived by crossing MSH-dr lines with Col-0 wild type, followed by selection for homozygous MSH1/MSH1 F2 plants and serial self-pollination. MSH1-epiF3 plants displayed phenotypic variation in plant growth, showing enhanced growth, larger rosette diameter, thicker floral stems and earlier flowering time. We carried bisulfite sequencing and uncover the methylome changes accompany the heritable MSH1-epi phenotypes that condition dramatic variation in plant growth.

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Small RNA sequencing of progenies of Col-0 grafted on msh1; Col-0 grafted on dcl2,3,4,msh1; msh1 mutant; dcl2,3,4,msh1 quadruple mutant in Arabidopsis [msh1_graft_sRNA-seq]

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