Unknown,Transcriptomics,Genomics,Proteomics

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Primary murine embryonic fibroblasts (MEFs): Cdk7 wild type versus Cdk7 KO


ABSTRACT: Transcriptional profiling of primary Cdk7lox/lox MEFs comparing Adeno-Cre vs Adeno-GFP infected controls. Samples were obtained 7 days after the Adeno-Cre mediated elimination of Cdk7. Goal was to determine the effects of Cdk7 elimination on RNA polII-mediated transcription. Two-condition experiment, Adeno-Cre vs. Adeno-GFP infected Cdk7lox/lox MEFs. Biological replicates: 10 independent primary MEF preparations.

ORGANISM(S): Mus musculus

SUBMITTER: David Santamaría 

PROVIDER: E-GEOD-36050 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Genetic inactivation of Cdk7 leads to cell cycle arrest and induces premature aging due to adult stem cell exhaustion.

Ganuza Miguel M   Sáiz-Ladera Cristina C   Cañamero Marta M   Gómez Gonzalo G   Schneider Ralph R   Blasco María A MA   Pisano David D   Paramio Jesús M JM   Santamaría David D   Barbacid Mariano M  

The EMBO journal 20120413 11


Cyclin-dependent kinase (Cdk)7, the catalytic subunit of the Cdk-activating kinase (CAK) complex has been implicated in the control of cell cycle progression and of RNA polymerase II (RNA pol II)-mediated transcription. Genetic inactivation of the Cdk7 locus revealed that whereas Cdk7 is completely dispensable for global transcription, is essential for the cell cycle via phosphorylation of Cdk1 and Cdk2. In vivo, Cdk7 is also indispensable for cell proliferation except during the initial stages  ...[more]

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