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Small molecules enable highly efficient neuronal conversion of human fibroblasts

ABSTRACT: Forced expression of pro-neural transcription factors was shown to mediate direct neuronal conversion of human fibroblasts. Since neurons are postmitotic, the conversion efficiency represents an important parameter. Here we present a minimalist approach combining two factor neuronal programming with small molecule-based inhibition of GSK3ß and SMAD signaling, which gives rise to functional neuron-like cells (iNs) of various neurotransmitter phenotypes with an overall yield of up to >200% and a final neuronal purity of up to >80%. Timcourse of reprogramming of fibroblasts towards an neuronal phenotype in two independent fibroblast lines

ORGANISM(S): Homo sapiens

SUBMITTER: Franz-Josef Müller

PROVIDER: E-GEOD-36246 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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