Small molecules enable highly efficient neuronal conversion of human fibroblasts
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ABSTRACT: Forced expression of pro-neural transcription factors was shown to mediate direct neuronal conversion of human fibroblasts. Since neurons are postmitotic, the conversion efficiency represents an important parameter. Here we present a minimalist approach combining two factor neuronal programming with small molecule-based inhibition of GSK3ß and SMAD signaling, which gives rise to functional neuron-like cells (iNs) of various neurotransmitter phenotypes with an overall yield of up to >200% and a final neuronal purity of up to >80%.
ORGANISM(S): Homo sapiens
PROVIDER: GSE36246 | GEO | 2012/04/08
SECONDARY ACCESSION(S): PRJNA153057
REPOSITORIES: GEO
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