Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Mårten Fryknäs
PROVIDER: E-GEOD-36666 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Karlsson Henning H Fryknäs Mårten M Larsson Rolf R Nygren Peter P
Experimental cell research 20120402 13
Clinically relevant in vitro methods are needed to identify new cancer drugs for solid tumors. We report on a new 3-D spheroid cell culture system aimed to mimic the properties of solid tumors in vivo. The colon cancer cell lines HCT-116 wt and HCT-116 wt/GFP were grown as monolayers and for 3 or 6 days on 96-well NanoCulture® plates to form spheroids. Expression of surface markers, genes and hypoxia were assessed to characterize the spheroids and drug induced cytotoxicity was evaluated based on ...[more]