Unknown,Transcriptomics,Genomics,Proteomics

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Spheroid model system


ABSTRACT: Microarray based mRNA profiling was used to charactarize and compare the gene expression in cells grown as monolayer or spheroids. Microarray based mRNA profiling was used to charactarize and compare the gene expression in cells grown as monolayer or spheroids.

ORGANISM(S): Homo sapiens

SUBMITTER: MÃ¥rten Fryknäs 

PROVIDER: E-GEOD-36666 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Loss of cancer drug activity in colon cancer HCT-116 cells during spheroid formation in a new 3-D spheroid cell culture system.

Karlsson Henning H   Fryknäs Mårten M   Larsson Rolf R   Nygren Peter P  

Experimental cell research 20120402 13


Clinically relevant in vitro methods are needed to identify new cancer drugs for solid tumors. We report on a new 3-D spheroid cell culture system aimed to mimic the properties of solid tumors in vivo. The colon cancer cell lines HCT-116 wt and HCT-116 wt/GFP were grown as monolayers and for 3 or 6 days on 96-well NanoCulture® plates to form spheroids. Expression of surface markers, genes and hypoxia were assessed to characterize the spheroids and drug induced cytotoxicity was evaluated based on  ...[more]

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