Dataset Information

Proteomics on colorectal cancer MCTS upon OXPHOS inhibition

ABSTRACT: Previous high throughput gene expression profiling combined with a HCT116 colon cancer tumor spheroid-based drug-screening assay identified context-dependent treatment responses to OXPHOS inhibitors resulting in a shunting towards the mevalonate pathway. To complement these findings with a characterization of the proteome we use mass-spectrometry based proteomics to investigate protein profiles and context-dependent treatment responses in PMCTS and QMCTS compared to monolayer cells.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell

DISEASE(S): Colon Cancer

SUBMITTER: Julia Steinmetz

LAB HEAD: Kim Kultima

PROVIDER: PXD016495 | Pride | 2020-07-29

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Julia_TMT_160428_Sets_04_fr_19_160503173419.raw	Raw
	Julia_TMT_160428_Sets_04_fr_20.raw	Raw
	Julia_TMT_160428_Sets_04_fr_20_160503192854.raw	Raw
	PD_Resultfile_6h24h_Allpep.pdResult	Other
	RAW_CTRL_Sets_C_B11.raw	Raw

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Publications

Descriptive Proteome Analysis to Investigate Context-Dependent Treatment Responses to OXPHOS Inhibition in Colon Carcinoma Cells Grown as Monolayer and Multicellular Tumor Spheroids.

Steinmetz Julia J Senkowski Wojciech W Lengqvist Johan J Rubin Jenny J Ossipova Elena E Herman Stephanie S Larsson Rolf R Jakobsson Per-Johan PJ Fryknäs Mårten M Kultima Kim K

ACS omega 20200706 28

We have previously identified selective upregulation of the mevalonate pathway genes upon inhibition of oxidative phosphorylation (OXPHOS) in quiescent cancer cells. Using mass spectrometry-based proteomics, we here investigated whether these responses are corroborated on the protein level and whether proteomics could yield unique insights into context-dependent biology. HCT116 colon carcinoma cells were cultured as monolayer cultures, proliferative multicellular tumor spheroids (P-MCTS), or qui ...[more]

PMID: 32715210

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