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Expression data from mouse lungs exposed in-utero and/or as an adult to second-hand smoke (SHS)

ABSTRACT: Second-hand smoke (SHS) exposure during pregnancy has adverse effects on offspring. We used microarrays to characterize the gene expression changes caused by in-utero exposure and adult exposure to SHS in adult mouse lungs. Left lungs from Balb/c male mice were collected at 15 weeks of age for RNA extraction and hybridization on Affymetrix mouse 430 2.0 microarrays. Based on their smoke exposure status, there are 4 groups of mice, each exposed in-utero to filtered-air or SHS and as an adult to filtered-air or SHS. We extracted RNA from 4 animals from each group for microarray analysis (N = 16 samples).

ORGANISM(S): Mus musculus

SUBMITTER: Rui Xiao

PROVIDER: E-GEOD-36810 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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In utero exposure to second-hand smoke aggravates adult responses to irritants: adult second-hand smoke.

Xiao Rui R Perveen Zakia Z Paulsen Daniel D Rouse Rodney R Ambalavanan Namasivayam N Kearney Michael M Penn Arthur L AL

American journal of respiratory cell and molecular biology 20120906 6

In utero exposure to second-hand smoke (SHS) is associated with exacerbated asthmatic responses in children. We tested the hypothesis that in utero SHS will aggravate the lung responses of young adult mice re-exposed to SHS. We exposed Balb/c mice in utero to SHS (S) or filtered air (AIR; A), and re-exposed the male offspring daily from 11-15 weeks of age to either SHS (AS and SS) or AIR (AA and SA). After the adult exposures, we analyzed samples of bronchoalveolar lavage fluid (BALF), examined ...[more]

PMID: 22962063

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Expression data from mouse lungs, exposed in-utero to second-hand smoke (SHS) and challenged with ovalbumin (OVA) as adults.

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