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Tracing the conversion process for primordial germ cells to pluripotent stem cells

ABSTRACT: The overall gene expression profiles revealed by this system provide novel insight into how pluripotency is acquired in germ-committed cells. In the study presented here, we employed FACS sorting to purify pluripotent candidate cells during the culture period

ORGANISM(S): Mus musculus

SUBMITTER: Satoshi Kondo

PROVIDER: E-GEOD-37261 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tracing the conversion process from primordial germ cells to pluripotent stem cells in mice.

Nagamatsu Go G Kosaka Takeo T Saito Shigeru S Takubo Keiyo K Akiyama Hideo H Sudo Tetsuo T Horimoto Katsuhisa K Oya Mototsugu M Suda Toshio T

Biology of reproduction 20120614 6

To understand mechanisms underlying acquisition of pluripotency, it is critical to identify cells that can be converted to pluripotent stem cells. For this purpose, we focused on unipotent primordial germ cells (PGCs), which can be reprogrammed into pluripotent embryonic germ (EG) cells under defined conditions. Treatment of PGCs with combinations of signaling inhibitors, including inhibitors of MAP2K (MEK), GSK3B (GSK-3beta), and TGFB (TGFbeta) type 1 receptors, induced cells to enter a pluripo ...[more]

PMID: 22423052

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