Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptional profile of rve8 in circadian time course

ABSTRACT: Analysis of Arabidopsis Columbia and rve8-1, a longer period mutant, in circadian time course (ZT72-116). Results provide insight into where RVE8 functions in the clock system and the targets regulated by RVE8. Arabidopsis Col and rve8-1 seedlings (~50 plants/ per sample) were grown in 12 hours light/ 12 hours dark for 7 days before releasing to the constant light. After 72 hours in the constant light, samples were harvested every 4 hours for 2 days. RNA was extracted, labeled and hybridized onto Agronomics1 tiling arrays.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Polly Hsu

PROVIDER: E-GEOD-37278 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Circadian phase has profound effects on differential expression analysis.

Hsu Polly Yingshan PY Harmer Stacey L SL

PloS one 20121120 11

Circadian rhythms are physiological and behavioral cycles with a period of approximately 24 hours that are generated by an endogenous clock, or oscillator. Found in diverse organisms, they are precisely controlled and provide growth and fitness benefits. Numerous microarray studies examining circadian control of gene expression have reported that a substantial fraction of the genomes of many organisms is clock-controlled. Here we show that a long-period mutant in Arabidopsis, rve8-1, has a globa ...[more]

PMID: 23185460

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Project description:Investigation of whole genome gene expression level changes in response to different light conditions of the T. reesei QM9414 deletion strains delta-blr1, delta-blr2 and delta env1 cultivated on 1% microcrystalline cellulose. Perception and proper interpretation of environmental signals is crucial for survival in any natural habitat. Although the biotechnological workhorse Trichoderma reesei (Hypocrea jecorina) is predominantly known for its capability of efficient plant cell wall degradation, recent studies show that it has not lost its evolutionary heritage. Transmission of nutrient signals via the heterotrimeric G protein pathway has been shown to be influenced by light. We show that this interconnection is mainly established by the light regulatory protein ENV1 and the phosducin-like protein PhLP1 via mutual transcriptional regulation and influence on GNB1 (G protein beta subunit) function. ENV1 thereby exerts a more severe effect on gene transcription than BLR1 or BLR2. Lack of either one of the photoreceptors or PhLP1, GNB1 or GNG1 leads to a partial shutdown of processes upregulated in light, indicating that heterotrimeric G protein signalling exerts its major function in light and is a target of the light response machinery. Consequently, signals transmitted via the G protein pathway are of different relevance in light and darkness. Investigation of regulation of glycoside hydrolases as one of the major output pathways of this mechanism revealed that 79% of all genes belonging to this group, representing all GH-families available in T. reesei, are potentially responsive to light. We conclude that ENV1 is a key factor in connecting nutrient signalling with light response and establishes a signalling output pathway independent of BLR1 and BLR2. We used two biological replicates of three T. reesei strains (delta-blr1, delta-blr2 and delta-env1), cultivated in constant light (LL, 1800 lux) or constant darkness (DD) on microcrystalline cellulose. The strains used in this study were cultivated, hybridized and analyzed together with strains and samples from GSE27581; the corresponding wild-type strain QM9414 samples have accession numbers GSM683732, GSM683733, GSM683734 and GSM683735.

Dataset Information

Transcriptional profile of rve8 in circadian time course

Publications

Circadian phase has profound effects on differential expression analysis.

Similar Datasets

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