Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from CD44loCD8+ T cells of sanroque versus wild-type mice

ABSTRACT: We used microarrays to detect the primary changes caused by the 'san' mutation in Roquin gene by comparing the gene expression profiles of naive (CD44lo) CD8+ T cell population. Sorted CD44loCD8+ T cells of age-matched sanroque (Roquinsan/san) and wild-type (Roquin+/+) mice in C57BL/6 background were subjected to RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Pheh Ping Chang

PROVIDER: E-GEOD-37319 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Breakdown in repression of IFN-γ mRNA leads to accumulation of self-reactive effector CD8+ T cells.

Chang Pheh-Ping PP Lee Sau K SK Hu Xin X Davey Gayle G Duan Guowen G Cho Jae-Ho JH Karupiah Guna G Sprent Jonathan J Heath William R WR Bertram Edward M EM Vinuesa Carola G CG

Journal of immunology (Baltimore, Md. : 1950) 20120608 2

Tight regulation of virus-induced cytotoxic effector CD8(+) T cells is essential to prevent immunopathology. Naturally occurring effector CD8(+) T cells, with a KLRG1(hi) CD62L(lo) phenotype typical of short-lived effector CD8(+) T cells (SLECs), can be found in increased numbers in autoimmune-prone mice, most notably in mice homozygous for the san allele of Roquin. These SLEC-like cells were able to trigger autoimmune diabetes in a susceptible background. When Roquin is mutated (Roquin(san)), e ...[more]

PMID: 22685317

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Project description:It has been recognised for a long time that cell surface glycans plays a vital role in the biological process and their altered form can lead to cancer. However, the molecular details and regulatory mechanism between the glycans and cancer is yet not clear. Over the past decade mass spectrometry-based techniques has become a prominent method for analysing glycans especially N-glycan matrix assisted laser desorption/ionisation mass spectrometry imaging (MALDI MSI). It is a powerful technique that combines mass spectrometry with histology, enabling the visualisation and label free detection of N-linked glycans on a single tissue section. Here, we carried out N-glycan MALDI MSI on six endometrial cancer (EC) formalin fixed paraffin embedded (FFPE) tissue sections including the adjacent normal endometrium, and tissue microarrays (TMA) consisting of 8 EC patients with lymph node metastasis (LNM) and 20 without LNM. By doing that several m/z values were detected that can significantly distinguish normal endometrium from cancerous. Also, detected a m/z value that can discriminate the primary tumour with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS). Overall, we observed higher abundance of oligomannose in tumour regions compared to normal with AUC ranges from 0.85-0.99. Whereas, complex N-glycans were detected in lower abundance with AUC ranges from 0.03-0.28. Comparison of N-glycans between the primary tumours with LNM and without LNM indicates reduced abundance of a complex core-fucosylated N-glycan in patients with metastasis relative to without. In summary, N-glycan MALDI MSI can be used to characterize the cancerous endometrium from the normal, also patients with LNM from those without. Identification of those discriminative m/z values was performed using porous graphitized carbon liquid chromatography tandem mass spectrometry (PGC-LC-MS/MS).

Dataset Information

Expression data from CD44loCD8+ T cells of sanroque versus wild-type mice

Publications

Breakdown in repression of IFN-γ mRNA leads to accumulation of self-reactive effector CD8+ T cells.

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