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Expression data of CD44loCD8+ T cells from sanroque versus wild-type mice

ABSTRACT: We used microarrays to detect the primary changes caused by the 'san' mutation in Roquin gene by comparing the gene expression profiles of naive (CD44lo) CD8+ T cell population.

ORGANISM(S): Mus musculus

PROVIDER: GSE37319 | GEO | 2012/04/17

SECONDARY ACCESSION(S): PRJNA159571

REPOSITORIES: GEO

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Expression data from CD44loCD8+ T cells of sanroque versus wild-type mice

Project description:We used microarrays to detect the primary changes caused by the 'san' mutation in Roquin gene by comparing the gene expression profiles of naive (CD44lo) CD8+ T cell population. Sorted CD44loCD8+ T cells of age-matched sanroque (Roquinsan/san) and wild-type (Roquin+/+) mice in C57BL/6 background were subjected to RNA extraction and hybridization on Affymetrix microarrays.

2012-04-16 | E-GEOD-37319 | biostudies-arrayexpress

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Project description:During acute viral infections, naïve CD8+ T cells differentiate into effector CD8+ T cells and, after viral control, into memory CD8+ T cells. Memory CD8+ T cells are highly functional, proliferate rapidly upon reinfection and persist long-term without antigen. In contrast, during chronic infections, CD8+ T cells become “exhausted” and have poor effector function, express multiple inhibitory receptors, possess low proliferative capacity, and cannot persist without antigen. To compare the development of functional memory T cells with poorly functional exhausted T cells, we generated longitudinal transcriptional profiles for each. Naive CD44Lo CD8+ T cells were isolated and sorted from uninfected C57BL/6 mice and H2-Db GP33-specific CD8+ T cells were sorted using MHC-I tetramers at d6, 8, 15, and 30 p.i. with either LCMV Arm or LCMV clone 13. RNA from these CD8+ T cells was processed, amplified, labeled, and hybridized to Affymetrix GeneChip MoGene 1.0 st microarrays

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Comparison of mouse naïve (CD44lo) CD8+ T cells sorted into the highest and lowest 20% with respect to CD5

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