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Transcription profiling by array of TET2 wild type and TET2 mutant patients with diffuse large B-cell derived lymphoma

ABSTRACT: Global gene expression in TET2 mutant and Wild type patients. We performed an integrated analysis of global DNA methylation and gene expression data to investigate the effects of DNA hypermethylation on gene expression. Total RNA was isolated from 4 TET2mut and 5 TET2wt were isolated using standard RNAeasy kit protocol (Qiagen). Their gene expression profiles were obtained using the[HuGene-1_0-st] Affymetrix.

ORGANISM(S): Homo sapiens

SUBMITTER: Vasu Punj

PROVIDER: E-GEOD-37363 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide profiling identifies a DNA methylation signature that associates with TET2 mutations in diffuse large B-cell lymphoma.

Asmar Fazila F Punj Vasu V Christensen Jesper J Pedersen Marianne T MT Pedersen Anja A Nielsen Anders B AB Hother Christoffer C Ralfkiaer Ulrik U Brown Peter P Ralfkiaer Elisabeth E Helin Kristian K Grønbæk Kirsten K

Haematologica 20130705 12

The discovery that the Ten-Eleven Translocation (TET) hydroxylases cause DNA demethylation has fundamentally changed the notion of how DNA methylation is regulated. Clonal analysis of the hematopoetic stem cell compartment suggests that TET2 mutations can be early events in hematologic cancers and recent investigations have shown TET2 mutations in diffuse large B-cell lymphoma. However, the detection rates and the types of TET2 mutations vary, and the relation to global methylation patterns has ...[more]

PMID: 23831920

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