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Sequential stage specific reporter line analysis (SSRLA) of 3 BAC transgenic mESC lines

ABSTRACT: FACS purified cells from differentiation day 14-15 cells from 3 BAC transgenic mESC lines: Hes::GFP (early), Nurr1::GFP (mid), and Pitx3::YFP (late) DA neuron development reporter lines All three lines were differentiated towards the midbrain dopamine phenotype, and FACS purification was performed at D14-15, and then subject to global transcriptome analysis

ORGANISM(S): Mus musculus

SUBMITTER: Jeffrey Zhao

PROVIDER: E-GEOD-37446 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of embryonic stem cell-derived midbrain dopaminergic neurons for engraftment.

Ganat Yosif M YM Calder Elizabeth L EL Kriks Sonja S Nelander Jenny J Tu Edmund Y EY Jia Fan F Battista Daniela D Harrison Neil N Parmar Malin M Tomishima Mark J MJ Rutishauser Urs U Studer Lorenz L

The Journal of clinical investigation 20120702 8

Embryonic stem cells (ESCs) represent a promising source of midbrain dopaminergic (DA) neurons for applications in Parkinson disease. However, ESC-based transplantation paradigms carry a risk of introducing inappropriate or tumorigenic cells. Cell purification before transplantation may alleviate these concerns and enable identification of the specific DA neuron stage most suitable for cell therapy. Here, we used 3 transgenic mouse ESC reporter lines to mark DA neurons at 3 stages of differentia ...[more]

PMID: 22751106

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