Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Oncogenic BRAF regulates oxidative metabolism via PGC1alpha and MITF


ABSTRACT: We evaluated two matched human cell lines derived from primary melanocytes, termed pmel+BRAF(V600E) and pmel+BRAF(V600E)+MITF. These cells are therefore isogenic with the exception of the expression of MITF. Gene Set Enrichment Analysis of microarray data identified a highly significant induction of oxidative phosphorylation gene set in MITF expressing cells compared to control cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Hans WIDLUND 

PROVIDER: E-GEOD-38007 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


Activating mutations in BRAF are the most common genetic alterations in melanoma. Inhibition of BRAF by small molecules leads to cell-cycle arrest and apoptosis. We show here that BRAF inhibition also induces an oxidative phosphorylation gene program, mitochondrial biogenesis, and the increased expression of the mitochondrial master regulator, PGC1α. We further show that a target of BRAF, the melanocyte lineage factor MITF, directly regulates the expression of PGC1α. Melanomas with activation of  ...[more]

Similar Datasets

2011-09-13 | E-GEOD-25173 | biostudies-arrayexpress
2011-08-30 | E-GEOD-25172 | biostudies-arrayexpress
2014-01-14 | E-GEOD-50649 | biostudies-arrayexpress
2014-09-19 | E-GEOD-61544 | biostudies-arrayexpress
2014-09-19 | GSE61544 | GEO
2019-11-12 | PXD010782 | Pride
2022-02-28 | PXD010665 | Pride
2023-03-11 | PXD030527 | Pride
2014-01-14 | GSE50649 | GEO
2014-01-14 | E-GEOD-50681 | biostudies-arrayexpress