Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

41 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of S. cerevisiae where Histone H2B was mutated to give H2B^3-37 to identify genes regulated by the H2B N-terminal domain

ABSTRACT: Histone H2B was mutated to give H2B^3-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Experiment Overall Design: mutant compared to wild-type, 3 replicates

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick

PROVIDER: E-GEOD-3804 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Deciphering the roles of the histone H2B N-terminal domain in genome-wide transcription.

Parra Michael A MA Kerr David D Fahy Deirdre D Pouchnik Derek J DJ Wyrick John J JJ

Molecular and cellular biology 20060501 10

Histone N-terminal domains are frequent targets of posttranslational modifications. Multiple acetylated lysine residues have been identified in the N-terminal domain of H2B (K6, K11, K16, K17, K21, and K22), but little is known about how these modifications regulate transcription. We systematically mutated the N-terminal domain of histone H2B, both at known sites of lysine acetylation and elsewhere, and characterized the resulting changes in genome-wide expression in each mutant strain. Our resu ...[more]

PMID: 16648479

Similar Datasets

Transcription profiling of S. cerevisiae H2B K6,11,16,17,21,22G mutant vs. wild type to investigate the role of H2B N-terminal domain in transcription

Project description:Histone H2B was mutated to give H2B K6,11,16,17,21,22G. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal acetylated lysine residues. Experiment Overall Design: mutant compared to wild-type, 3 replicates

2008-06-12 | E-GEOD-3803 | biostudies-arrayexpress

Transcription profiling of S. cerevisiae H2B^3-32 mutants to investigate the role of hstone H2B N-terminal domain in genome wide transcription

Project description:Histone H2B was mutated to give H2B^3-32. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Experiment Overall Design: mutant compared to wild-type, 3 replicates

2008-06-12 | E-GEOD-3802 | biostudies-arrayexpress

Transcription profiling of S. cerevisiae histone H2Bdelat30-37 mutants vs wild type to determine the role of H2B N terminal domaon in transcription

Project description:Histone H2B was mutated to give H2B^30-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B HBR domain. Experiment Overall Design: mutant compared to wild-type, 3 replicates

2008-06-12 | E-GEOD-3805 | biostudies-arrayexpress

Histone H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37 mutations

Project description:Histone H2B was mutated to give H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B HBR domain. This SuperSeries is composed of the SubSeries listed below.

2006-04-08 | GSE3806 | GEO

Histone H2B^30-37

2006-04-08 | GSE3805 | GEO

Histone H2B^3-37

Project description:Histone H2B was mutated to give H2B^3-37. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal domain. Keywords: repeat

2006-04-08 | GSE3804 | GEO

Histone H2B R102A

Project description:Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by histone H2B R102A mutant. Comparison of genes whose mRNA levels are affected in a histone H2B R102A mutant yeast strain compared to wild-type yeast strain.

2010-03-09 | E-GEOD-15202 | biostudies-arrayexpress

Histone H2B K111A

Project description:Total RNA from three replicate cultures of wild-type and mutant strains was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by histone H2B K111A mutant. Comparison of genes whose mRNA levels are affected in a histone H2B K111A mutant yeast strain compared to wild-type yeast strain.

2010-03-09 | E-GEOD-15203 | biostudies-arrayexpress

Histone H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37 mutations

Project description:Histone H2B^3-32, H2B K->G, H2B^3-37, and H2B^30-37 mutations

| PRJNA94007 | ENA

A Basic Domain in the Histone H2B N-terminal Tail is Important for Nucleosome Assembly by FACT

Project description:Nucleosome assembly in vivo requires assembly factors, such as histone chaperones, to bind to histones and mediate their deposition onto DNA. In yeast, the essential histone chaperone FACT (FAcilitates Chromatin Transcription) functions in nucleosome assembly and H2A-H2B deposition during transcription elongation and DNA replication. Recent studies have identified candidate histone residues that mediate FACT binding to histones, but it is not known which histone residues are important for FACT to deposit histones onto DNA during nucleosome assembly. In this study, we report that the histone H2B repression (HBR) domain within the H2B N-terminal tail is important for histone deposition by FACT. Deletion of the HBR domain causes significant defects in histone occupancy in the yeast genome, particularly at HBR-repressed genes, and a pronounced increase in H2A-H2B dimers that remain bound to FACT in vivo. Moreover, the HBR domain is required for purified FACT to efficiently assemble recombinant nucleosomes in vitro. We propose that the interaction between the highly basic HBR domain and DNA plays an important role in stabilizing the nascent nucleosome during the process of histone H2A-H2B deposition by FACT. 6 samples, 2 inputs and 4 ChIP samples for histone H2B (2 for wild-type and 2 for an H2B â30-37 mutant)

2016-08-10 | E-GEOD-80831 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data